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Journal of Bacteriology, April 2005, p. 2551-2554, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2551-2554.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Structural and Genetic Characterization of the Shigella boydii Type 10 and Type 6 O Antigens

Sof'ya N. Senchenkova,1 Lu Feng,2,3 Jinghua Yang,2 Alexander S. Shashkov,1 Jiansong Cheng,2 Dan Liu,3 Yuriy A. Knirel,1 Peter R. Reeves,4 Qi Jin,5 Qiang Ye,6 and Lei Wang2,3*

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation,1 School of Molecular and Microbial Biosciences (G08), University of Sydney, Sydney, New South Wales, Australia,4 TEDA School of Biology Sciences and Biotechnology, Nankai University, TEDA,2 Tianjin State Laboratory of Microbial Functional Genomics, TEDA College, Nankai University, Tianjin,3 State Key Laboratory for Molecular Virology and Genetic Engineering,5 National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China6

Received 20 July 2004/ Accepted 20 December 2004

Comparison of the O antigens of Shigella boydii types 10 and 6 by chemical analysis and nuclear magnetic resonance spectroscopy showed that their structures are similar, with the only difference being the presence or absence of D-ribofuranose, which is the immunodominant sugar in S. boydii type 10. In S. boydii type 6, a residue previously reported as {alpha}-D-GlcpA, was shown to be ß-D-GlcpA as in S. boydii type 10. S. boydii types 10 and 6 are reported not to cross-react serologically, and the role of D-ribofuranose in the specificity of S. boydii was confirmed by making a mutant of type 10 that lacked D-ribofuranose. However, S. boydii type 11, which has a D-ribofuranose but with different linkage does show cross-reaction with type 10. The O-antigen gene loci of S. boydii types 10 and 6 were shown to be virtually identical except that orf8 (wbaM), which was confirmed as the ribofuranosyltransferase gene, is interrupted by IS629 in type 6. Therefore, it is proposed that the O-antigen gene cluster of S. boydii type 6 was derived from type 10 by an IS element insertion.

* Corresponding author. Mailing address: TEDA School of Biology Sciences and Biotechnology, Nankai University, 23# Hongda St., TEDA, Tianjin 300457, China. Phone: 86-22-66229588. Fax: 86-22-66229596. E-mail: wanglei{at}nankai.edu.cn.

Journal of Bacteriology, April 2005, p. 2551-2554, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2551-2554.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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