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Journal of Bacteriology, November 2008, p. 7559-7566, Vol. 190, No. 22
0021-9193/08/$08.00+0     doi:10.1128/JB.00470-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Possible Extended Family of Regulators of Sigma Factor Activity in Streptomyces coelicolor{triangledown} ,{dagger}

Eun Sook Kim,1 Ju Yeon Song,1 Dae Wi Kim,1 Keith F. Chater,2 and Kye Joon Lee1*

School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, South Korea,1 Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom2

Received 7 April 2008/ Accepted 2 September 2008

SCO4677 is one of a large number of similar genes in Streptomyces coelicolor that encode proteins with an HATPase_c domain resembling that of anti-sigma factors such as SpoIIAB of Bacillus subtilis. However, SCO4677 is not located close to genes likely to encode a cognate sigma or anti-anti-sigma factor. SCO4677 was found to regulate antibiotic production and morphological differentiation, both of which were significantly enhanced by the deletion of SCO4677. Through protein-protein interaction screening of candidate sigma factor partners using the yeast two-hybrid system, SCO4677 protein was found to interact with the developmentally specific {sigma}F, suggesting that it is an antagonistic regulator of {sigma}F. Two other proteins, encoded by SCO0781 and SCO0869, were found to interact with the SCO4677 anti-{sigma}F during a subsequent global yeast two-hybrid screen, and the SCO0869-SCO4677 protein-protein interaction was confirmed by coimmunoprecipitation. The SCO0781 and SCO0869 proteins resemble well-known anti-anti-sigma factors such as SpoIIAA of B. subtilis. It appears that streptomycetes may possess an extraordinary abundance of anti-sigma factors, some of which may influence diverse processes through interactions with multiple partners: a novel feature for such regulatory proteins.

* Corresponding author. Mailing address: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea. Phone: 82 2 880 6705. Fax: 82 2 882 9956. E-mail: lkj12345{at}snu.ac.kr

{triangledown} Published ahead of print on 12 September 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2008, p. 7559-7566, Vol. 190, No. 22
0021-9193/08/$08.00+0     doi:10.1128/JB.00470-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Parashar, A., Colvin, K. R., Bignell, D. R. D., Leskiw, B. K. (2009). BldG and SCO3548 Interact Antagonistically To Control Key Developmental Processes in Streptomyces coelicolor. J. Bacteriol. 191: 2541-2550 [Abstract] [Full Text]  


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