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Journal of Bacteriology, December 2008, p. 7666-7674, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.00868-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Two-Component Regulatory System Integrates Redox State and Population Density Sensing in Pseudomonas putida{triangledown} ,{dagger}

Regina Fernández-Piñar, Juan Luis Ramos, José Juan Rodríguez-Herva, and Manuel Espinosa-Urgel*

Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

Received 25 June 2008/ Accepted 18 September 2008

A two-component system formed by a sensor histidine kinase and a response regulator has been identified as an element participating in cell density signal transduction in Pseudomonas putida KT2440. It is a homolog of the Pseudomonas aeruginosa RoxS/RoxR system, which in turn belongs to the RegA/RegB family, described in photosynthetic bacteria as a key regulatory element. In KT2440, the two components are encoded by PP_0887 (roxS) and PP_0888 (roxR), which are transcribed in a single unit. Characterization of this two-component system has revealed its implication in redox signaling and cytochrome oxidase activity, as well as in expression of the cell density-dependent gene ddcA, involved in bacterial colonization of plant surfaces. Whole-genome transcriptional analysis has been performed to define the P. putida RoxS/RoxR regulon. It includes genes involved in sugar and amino acid metabolism and the sulfur starvation response and elements of the respiratory chain (a cbb3 cytochrome oxidase, Fe-S clusters, and cytochrome c-related proteins) or genes participating in the maintenance of the redox balance. A putative RoxR recognition element containing a conserved hexamer (TGCCAG) has also been identified in promoters of genes regulated by this two-component system.

* Corresponding author. Mailing address: Department of Environmental Protection, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, Granada 18008, Spain. Phone: 34 958 181600. Fax: 34 958 129600. E-mail: manuel.espinosa{at}eez.csic.es

{triangledown} Published ahead of print on 26 September 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2008, p. 7666-7674, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.00868-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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