This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mangalo, R. Articles by Wachsman, J. T. Search for Related Content PubMed PubMed Citation Articles by Mangalo, R. Articles by Wachsman, J. T.

 Previous Article  |  Next Article 

J Bacteriol. 1962 January; 83(1): 27-34
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

EFFECT OF 8-AZAGUANINE ON GROWTH AND VIABILITY OF BACILLUS MEGATERIUM

R. Mangalo¹ and J. T. Wachsman

^a Department of Microbiology, University of Illinois, Urbana, Illinois

ABSTRACT

MANGALO, R. (University of Illinois, Urbana) AND J. T. WACHSMAN. Effect of 8-azaguanine on growth and viability of Bacillus megaterium. J. Bacteriol. 83:27–34. 1962.—The addition of 8-azaguanine to exponentially growing cells of Bacillus megaterium results in an inhibition of growth after a lag of approximately 30 min. However, 8-azaguanine-2-C¹⁴ is incorporated into the nucleic acids in a linear fashion without a detectable lag. The inhibitory action is reversed by purines and their derivatives, but not by uridine, thymidine, or cytidine. 8-Azaguanine is bactericidal, especially under conditions where growth (ribonucleic acid synthesis) is possible. Growth in the presence of a complete amino acid mixture, either before or during exposure to 8-azaguanine, increases the rate of killing. Chloramphenicol has little or no effect on the bactericidal action of the analogue.

---

FOOTNOTES

¹ Permanent address: Institut Pasteur, Garches, Seine et Oise, France.

---

J Bacteriol. 1962 January; 83(1): 27-34
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.