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J Bacteriol. 1962 January; 83(1): 35-37
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

USE OF 8-AZAGUANINE FOR THE ISOLATION OF AUXOTROPHIC MUTANTS OF BACILLUS MEGATERIUM

J. T. Wachsman and R. Mangalo1

a Department of Microbiology, University of Illinois, Urbana, Illinois

ABSTRACT

WACHSMAN, J. T. (University of Illinois, Urbana) AND R. MANGALO. Use of 8-azaguanine for the isolation of auxotrophic mutants of Bacillus megaterium. J. Bacteriol. 83:35–37. 1962.—8-Azaguanine is bactericidal for Bacillus megaterium strain KM, primarily under conditions where growth is possible. This analogue has been used to enrich for auxotrophic mutants in ultraviolet-irradiated populations. The survivors were found to contain from 1.6 to 33.0% amino acid auxotrophs and from 19 to 29% pyrimidine nucleoside auxotrophs. Since many different biological systems are sensitive to 8-azaguanine, the use of this analogue to enrich for auxotrophic mutants may have a more general application than techniques currently employed.

FOOTNOTES

1 Permanent address: Institut Pasteur, Garches, Seine et Oise, France.

J Bacteriol. 1962 January; 83(1): 35-37
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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