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J Bacteriol. 1962 January; 83(1): 6-13
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

GLYCINE RESISTANCE IN AGROBACTERIUM TUMEFACIENS

Robert E. Beardsley

Manhattan College, New York, New York

ABSTRACT

BEARDSLEY, ROBERT E. (Manhattan College, New York, N. Y.). Glycine resistance in Agrobacterium tumefaciens. J. Bacteriol. 83:6–13. 1962.—The application of the fluctuation test of Luria and Delbrück to the distribution of glycine-resistant bacteria among cultures of Agrobacterium tumefaciens strain B6 indicates that resistance arises by mutation in the absence of glycine. On glycine-supplemented medium, additional resistant colonies arise during prolonged periods of incubation. Their appearance is proceded by L-form growth. In general, the number of generations over which glycine resistance is inherited in the absence of glycine is increased by serial transfers on the selection medium. In liquid medium containing glycine, sensitive bacteria form spheroplasts. Resistant bacteria continue to grow as rod forms. In the medium employed, spheroplasts are unstable.

J Bacteriol. 1962 January; 83(1): 6-13
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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