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J Bacteriol. 1962 January; 83(1): 76-84
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

STUDIES ON THE CARBOHYDRATE METABOLISM OF A GRAM-NEGATIVE ANAEROBE (BACTEROIDES SYMBIOSUS) USED IN THE CULTURE OF ENTAMOEBA HISTOLYTICA1

P. D. Bragg2 and Richard E. Reeves

a Department of Biochemistry, Louisiana State University, School of Medicine, New Orleans, Louisiana

ABSTRACT

BRAGG, P. D. (Louisiana State University, New Orleans) AND R. E. REEVES. Studies on the carbohydrate metabolism of a gram-negative anaerobe (Bacteroides symbiosus) used in the culture of Entamoeba histolytica. J. Bacteriol. 83:76–84. 1962—Resting cells of Bacteroides symbiosus have been shown to utilize glucose and several other monosaccharides. The fermentation of the sugars is mediated by demonstrable kinases except in the case of mannitol. The main end products of metabolism of glucose are CO2, H2, ethanol, and acetic, butyric, succinic, and lactic acids. Changes in the thiol used in the growth media produce different enzyme complements in the cells. Thus, cells grown with cysteine as the thiol are unable to metabolize glucosamine, whereas those grown with thiomalate rapidly degrade the amino sugar. The results of the enzyme assay and the results from experiments with C14-labelled glucose suggest that glucose is metabolized by resting cells mainly by the Embden-Myerhof pathway.

FOOTNOTES

2 Present address: Department of Biochemistry, University of British Columbia, Vancouver, B. C., Canada.

1 This investigation was conducted under the sponsorship of the Commission on Enteric Infections of the Armed Forces Epidemiological Board.

J Bacteriol. 1962 January; 83(1): 76-84
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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