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Journal of Bacteriology, April 2005, p. 2278-2285, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2278-2285.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Pasteurella multocida nrfE Gene Is Upregulated during Infection and Is Essential for Nitrite Reduction but Not for Virulence

David J. Boucher, Ben Adler,* and John D. Boyce

Australian Research Council Centre for Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Melbourne, Australia

Received 21 August 2004/ Accepted 14 December 2004

Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a previous study in which IVET was used with P. multocida, nrfE was identified as a gene that is preferentially expressed in vivo. In Escherichia coli, nrfE is part of the formate-dependent nitrite reductase system involved in utilizing available nitrite as an electron accepter during growth under anaerobic conditions. In this study, we constructed an isogenic P. multocida strain that was unable to reduce nitrite under either aerobic or anaerobic conditions, thereby demonstrating that P. multocida nrfE is essential for nitrite reduction. However, the nrfE mutant was still virulent in mice. Real-time reverse transcription-PCR analysis indicated that nrfE was regulated independently of nrfABCD by an independent promoter that is likely to be upregulated in vivo.

* Corresponding author. Mailing address: Australian Research Council Centre for Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Victoria 3800, Australia. Phone: 61 3 9905 4815. Fax: 61 3 9905 4811. E-mail: Ben.Adler{at}med.monash.edu.au.

Journal of Bacteriology, April 2005, p. 2278-2285, Vol. 187, No. 7
0021-9193/05/$08.00+0     doi:10.1128/JB.187.7.2278-2285.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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