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Journal of Bacteriology, November 2008, p. 7108-7116, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00855-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Nitrogen Control in Mycobacterium smegmatis: Nitrogen-Dependent Expression of Ammonium Transport and Assimilation Proteins Depends on the OmpR-Type Regulator GlnR{triangledown} ,{dagger}

Johannes Amon, Tanja Bräu, Aletta Grimrath, Eva Hänβler, Kristin Hasselt, Martina Höller, Nadja Jeβberger, Lisa Ott, Juraj Szököl, Fritz Titgemeyer, and Andreas Burkovski*

Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

Received 23 June 2008/ Accepted 28 July 2008

The effect of nitrogen regulation on the level of transcriptional control has been investigated in a variety of bacteria, such as Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, and Streptomyces coelicolor; however, until now there have been no data for mycobacteria. In this study, we found that the OmpR-type regulator protein GlnR controls nitrogen-dependent transcription regulation in Mycobacterium smegmatis. Based on RNA hybridization experiments with a wild-type strain and a corresponding mutant strain, real-time reverse transcription-PCR analyses, and DNA binding studies using cell extract and purified protein, the glnA (msmeg_4290) gene, which codes for glutamine synthetase, and the amtB (msmeg_2425) and amt1 (msmeg_6259) genes, which encode ammonium permeases, are controlled by GlnR. Furthermore, since glnK (msmeg_2426), encoding a PII-type signal transduction protein, and glnD (msmeg_2427), coding for a putative uridylyltransferase, are in an operon together with amtB, these genes are part of the GlnR regulon as well. The GlnR protein binds specifically to the corresponding promoter sequences and functions as an activator of transcription when cells are subjected to nitrogen starvation.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. Phone: 49 9131 85 28086. Fax: 49 9131 85 28082. E-mail: aburkov{at}biologie.uni-erlangen.de

{triangledown} Published ahead of print on 8 August 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2008, p. 7108-7116, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00855-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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