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Journal of Bacteriology, November 2008, p. 7170-7177, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00747-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Soluble NADH-Dependent Fumarate Reductase in the Reductive Tricarboxylic Acid Cycle of Hydrogenobacter thermophilus TK-6{triangledown}

Akane Miura, Masafumi Kameya,* Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi

Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

Received 26 May 2008/ Accepted 16 August 2008

Fumarate reductase (FRD) is an enzyme that reduces fumarate to succinate. In many organisms, it is bound to the membrane and uses electron donors such as quinol. In this study, an FRD from a thermophilic chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6, was purified and characterized. FRD activity using NADH as an electron donor was not detected in the membrane fraction but was found in the soluble fraction. The purified enzyme was demonstrated to be a novel type of FRD, consisting of five subunits. One subunit showed high sequence identity to the catalytic subunits of known FRDs. Although the genes of typical FRDs are assembled in a cluster, the five genes encoding the H. thermophilus FRD were distant from each other in the genome. Furthermore, phylogenetic analysis showed that the H. thermophilus FRD was located in a distinct position from those of known soluble FRDs. This is the first report of a soluble NADH-dependent FRD in Bacteria and of the purification of a FRD that operates in the reductive tricarboxylic acid cycle.

* Corresponding author. Mailing address: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone: (81)-3-5841-5143. Fax: (81)-3-5841-5272. E-mail: kxxbb274{at}ybb.ne.jp

{triangledown} Published ahead of print on 29 August 2008.

Journal of Bacteriology, November 2008, p. 7170-7177, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00747-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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